|
KMID : 1140120100150030241
|
|
Cancer Prevention Research
2010 Volume.15 No. 3 p.241 ~ p.247
|
|
|
Cytotoxicity and Apoptosis-induced Activity of Extracts from Tetrapanax papyriferus
|
|
Moon Seong-Hee
Lee Jung-Min
Kim Hyun-Jung
Kang Young-Kyoung
Park Hae-Ryong
|
|
|
Abstract
|
|
|
|
In this study, we used methanolic extracts of Tetrapanax papyriferus (TPM) to determine their anticancer activities on HT-29 human colon cancer cells. After TPM treatment, morphological changes, such as chromatin condensation, were observed in HT-29 cells. We confirmed cytotoxicity through cell biological assays after treatment with TPM at various concentrations. An MTT reduction assay revealed that TPM treatment at a concentration of 500 μg/ml yielded a cell viability of 39.4%. The findings indicated that strong anticancer activity restricted colony formation at TPM concentration greater than 10 μg/ml. In addition, we performed apoptosis in a series of experiments in order to determine the cell iological mechanism of TPM cytotoxicity. By Hoechst staining, we confirmed the formation of apoptotic bodies in cells treated with TPM. The results of flow cytometric analysis indicated that while the number of cells in the sub-G1 phase was 0.7% in the control group and 13.1% in the group treated with 250 μg/ml TPM, we could identify a high apoptotic effect. Based on these results, we could confirm that TPM induces apoptosis in cancer cells and has strong anticancer properties.
|
|
|
KEYWORD
|
|
|
Tetrapanax papyriferus, HT-29 cells, MTT reduction assay, Cytotoxicity, Apoptosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|